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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOLO, P. S.; SANTOS, A. S. de O. dos; MONTEIRO, C. M. O.; PERINOTTO, W. M. de S.; QUINELATO, S.; CAMARGO, M. G.; SÁ, F. A. de; ANGELO, I. da C.; MARTINS, M. F.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. |
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Afiliação: |
Patrícia Silva Golo, UFRRJ; Alessa Siqueira de Oliveira dos Santos; Caio Marcio Oliveira Monteiro, UFRRJ; Wendell Marcelo de Souza Perinotto, Universidade de Cuiabá; Simone Quinelato, Fundação Osvaldo Cruz; Mariana Guedes Camargo, UFRRJ; Fillipe Araujo de Sá, UFRJ; Isabele da Costa Angelo, UFRRJ; MARTA FONSECA MARTINS, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Vânia Rita Elias Pinheiro Bittencourt, UFRRJ. |
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Título: |
Lab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari: Ixodidae) infected with entomopathogenic nematode and fungus. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Parasitology Research, n. 115, p. 3459-3468, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC. MenosAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation... Mostrar Tudo |
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Palavras-Chave: |
Cattle tick; Protein bands. |
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Thesaurus Nal: |
biological control; gel electrophoresis; Heterorhabditis; Metarhizium. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
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100 1 $aGOLO, P. S.
245 $aLab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari$bIxodidae) infected with entomopathogenic nematode and fungus.$h[electronic resource]
260 $c2016
520 $aAbstract In the present study, lab-on-a-chip electrophoresis (LoaC) was suggested as an alternative method to the conventional polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) to analyze raw cell-free tick hemolymph. Rhipicephalus microplus females were exposed to the entomopathogenic fungus Metarhizium anisopliae senso latu IBCB 116 strain and/or to the entomopathogenic nematode Heterorhabditis indica LPP1 strain. Hemolymph from not exposed or exposed ticks was collected 16 and 24 h after exposure and analyze by SDS-PAGE or LoaC. SDS-PAGE yielded 15 bands and LoaC electrophoresis 17 bands. Despite the differences in the number of bands, when the hemolymph protein profiles of exposed or unexposed ticks were compared in the same method, no suppressing or additional bandswere detected among the treatments regardless themethod (i.e., SDS-PAGE or chip electrophoresis using the Protein 230 Kit®). The potential of LoaC electrophoresis to detect protein bands from tick hemolymph was considered more efficient in comparison to the detection obtained using the traditional SDS-PAGE method, especially when it comes to protein subunits heavier than 100 KDa. LoaC electrophoresis provided a very good reproducibility, and is much faster than the conventional SDS-PAGE method, which requires several hours for one analysis. Despite both methods can be used to analyze tick hemolymph composition, LoaC was consideredmore suitable for cell-free hemolymph protein separation and detection. LoaC hemolymph band percent data reported changes in key proteins (i.e., HeLp and vitellogenin) exceptionally important for tick embryogenesis. This study reported, for the first time, tick hemolymph protein profile using LoaC.
650 $abiological control
650 $agel electrophoresis
650 $aHeterorhabditis
650 $aMetarhizium
653 $aCattle tick
653 $aProtein bands
700 1 $aSANTOS, A. S. de O. dos
700 1 $aMONTEIRO, C. M. O.
700 1 $aPERINOTTO, W. M. de S.
700 1 $aQUINELATO, S.
700 1 $aCAMARGO, M. G.
700 1 $aSÁ, F. A. de
700 1 $aANGELO, I. da C.
700 1 $aMARTINS, M. F.
700 1 $aPRATA, M. C. de A.
700 1 $aBITTENCOURT, V. R. E. P.
773 $tParasitology Research$gn. 115, p. 3459-3468, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 8 | |
| 3. |  | SILVA, E. R.; MONTEIRO, C. M. O.; CAMARGO, M. G.; PRATA, M. C. de A.; FURLONG, J. Ação in vitro de heterorhabditis indica (Rhabditida: heterorhabditidae), isolado LPP1, sobre a biologia reprodutiva de femeas ingurgitadas de Rhipicephalus (Boophilus) microplus (Acari: ixodidae). In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | MARCHESINI, P.; GOLO, P.; PERINOTTO, W. M. S.; CAMARGO, M. G.; PRATA, M. C. de A.; MONTEIRO, C. M. O.; ANGELO, I. C.; BITTENCOURT, V. R. E. P. Perfil lipídico de corpo gorduroso de femeas de Rhipicephalus microplus infectadas por fungos e nematoides entomopatogênicos. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 20., 2018, Londrina. Anais. Londrina: CBPV, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | CAMARGO, M. G.; MARCIANO, A. F.; SÁ, F. A.; PERINOTTO, W. M. S.; QUINELATO, S.; GÔLO, P. S.; ANGELO, I. C.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. Commercial formulation of Metarhizium anisopliae for the control of Rhipicephalus microplus in a pen study. Veterinary Parasitology, n. 205, p. 271-276, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | CAMARGO, M. G.; MARCIANO, A. F.; SÁ, F. A.; PERINOTTO, W. M. S.; QUINELATO, S.; GOLO, P. S.; ANGELO, I. C.; PRATA, M. C. de A.; BITTERNCOURT, V. R. E. P. Use of a commercial Metarhizium anisopliae s.l. formulation to control Rhipicephalus microplus ticks in pen study. Mainz Germany: Society for invertebrate pathology, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | CAMARGO, M. G.; NOGUEIRA, M. R. S.; MARCIANO, A. F.; PERINOTTO, W. M. S.; COUTINHO-RODRIGUES, C. J. B.; SCOTT, F. B.; ANGELO, I. C.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. Metarhizium anisopliae for controlling Rhipicephalus microplus ticks under field conditions. Veterinary Parasitology, v. 223, p. 38-42, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | GOLO, P. S.; SANTOS, A. S. de O. dos; MONTEIRO, C. M. O.; PERINOTTO, W. M. de S.; QUINELATO, S.; CAMARGO, M. G.; SÁ, F. A. de; ANGELO, I. da C.; MARTINS, M. F.; PRATA, M. C. de A.; BITTENCOURT, V. R. E. P. Lab-on-a-chip and SDS-PAGE analysis of hemolymph protein profile from Rhipicephalus microplus (Acari: Ixodidae) infected with entomopathogenic nematode and fungus. Parasitology Research, n. 115, p. 3459-3468, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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